Decreased ovarian Reserve
PRP Therapy for Ovarian Rejuvenation: an emerging ray of hope
Ovarian Rejuvenation is a novel form of treatment that has turned the fertility clock for women anti-clockwise. It has helped resolve problems and elevate chances of naturally conceived pregnancies .
Under favourable biological conditions, Stem Cells are capable of transforming themselves into any type of cell in the human body, including eggs. The researchers in Harvard have conducted studies demonstrating presence of Stem Cells in the ovaries and their transformation into egg cells /mature oocytes.
Likewise, presence of Stem Cells have also been observed in the human ovary, so it is quite possible that they can be transformed into eggs using Growth Factors present in the individuals’ very own blood components such as the WBC’s and Platelets which can be injected into the ovary.
Injections of Growth Factors, that are used for this and several other treatments are prepared using patient’s own blood/ blood cells and is known as PRP (Platelet Rich Plasma) or PDGF (Platelet Derived Growth Factors) Therapy.
What are growth factors, how do they work and are they safe?
Growth Factors are naturally produced biological agents that are derived from the blood cells (platelets and white blood cells) that are released when the body undergoes an injury, in order to naturally repair the body’s tissues.
Do y’all remember bruising your knee sometime during your childhood and seeing a thick yellow substance appear over the injured area as a result of the injury? This yellow substance is what actually ceases bleeding, it is a combination of platelets, WBC’s (white blood cells), and growth factors that prevent further infection, and eventually result in the formation of new skin, replaces damaged blood vessels, repairs connective tissues and replaces nerves in place of those which were lost as a result of the injury.
Over the years, we’ve used PRP injections several times clinically for the treatment of soft and connective tissue injuries, as well as in cases of bone grafts. Various professional athletes use PRP therapy to speed up the healing process of their sports related injuries. It is also used by burn patients to help with skin grafts for non-surgical facelifts.
There are several research studies that both assert and contradict the efficacy of PRP therapy in its several and varied medical uses. However the highlight is that PRP therapy has not been challenged for its safety yet because it is prepared using an individual’s own blood, hence the chances of transmission of blood borne viruses such as Hepatitis or HIV is completely eliminated. Also, the chances of an allergic reaction is extremely unlikely since there are no synthetic chemicals involved either.
How is the ovarian rejuvenation procedure performed?
The ovarian rejuvenation procedure comprises of two steps:
PREPARATION OF PRP: During this step, a defined amount of blood is withdrawn from your vein. This derived blood is sent to the lab for further processing wherein we isolate your WBC’s (white blood cells) and platelets from the RBC’s (red blood cells) and serum via centrifugation. The preparation of PRP takes almost less than an hour.
RE-ADMINISTRATION OF PRP INTO THE TARGET AREA: During this stage of the ovarian rejuvenation process we administer the processed PRP into the ovaries. The major breakthrough in our technique is that we abide by a non-surgical, transvaginal ultrasound- guided approach to carry forward the procedure. The administration is performed under sedation using an anaesthetic agent called Propofol. As per our experience , our approach is certainly less invasive, much safer, and has a shorter, and definitely more comfortable recovery.
What are the potential risks and benefits of the procedure?
The ultimate benefit of this procedure is the higher number of chances of achieving pregnancy using one’s own eggs, which certainly wasn’t possible prior to this procedure. However, Since it is an innovative medical treatment, it must be clearly understood that whatsoever there is no 100% guarantee, stated or implied, that pregnancy, be it naturally, or as a result of any subsequent fertility procedure, will occur. The risks involved in the procedure are minimal. The possible side effects are pain after the procedure, which usually resolves in less than 2 hours with the help of pain medication, fever and in very rare cases slight internal bleeding. Although complications are rare, they may possibly result in hospitalisation.
Who are candidates for this procedure?
Any woman who is fit and is in a sound physical health and falls into one or more of the four patient categories mentioned below:
- Menopausal or perimenopausal women under the age of 50 years.
- Infertile women, over the age of 35 years with poor egg reserve and low AntiMullerian Hormone levels.
- Women under the age of 35 years, with poor egg reserve and low AntiMullerian Hormone levels.
- Women with premature ovarian failure (POF).
How is the ovarian regeneration procedure performed?
In case of women who do not get their periods, the procedure can be conducted at any given time. However, for women who do get periods, either regularly/ irregularly, the procedure is advisable to be conducted while they are at an early stage in their menstrual cycle, prior to the development of an egg follicle. The procedure can be conducted as early as the day of the initial evaluation.
AMH (antiMullerian hormone), FSH,LH and Estradiol levels are measured at monthly intervals in women who do not menstruate in order to analyse if the procedure has done its job and has regenerated new eggs in your ovaries. Incase of women who are witnessing menstrual flow AMH (antiMullerian hormone), FSH,LH and Estradiol levels are measured for a period of six months. As per studies, If the AMH levels rise, while the FSH,LH, and estradiol levels become lower, there is clear evidence of ovarian rejuvenation happening. Nevertheless, even if slight ovarian rejuvenation is recorded, it does not justify that pregnancy may necessarily occur, since there are a lot of chances that other factors might interfere with natural conception and influence the overall results. The researchers need to do some further analysis in order to ensure that the resulting eggs are absolutely fine.
PRP has also showcased positive results in case of women who have a uterus that embryos find difficult to attach to reason being formation/presence of cysts, scarring from miscarriages or a thin uterine lining (Thin Endometrium). Women who seek fertility treatment often benefit in case they’re witnessing either of these problems. The trials and studies conducted so far, give hope that PRP Therapy for ovarian rejuvenation will definitely prove to be a life changing therapeutic option for women who’re suffering from age related infertility.
What are results derived so far?
PRP Therapy for Ovarian Rejuvenation has a positive patient feedback and great success rate. This has encouraged us to prepare and offer PRP injections for several other sexual dysfunctions such as Vaginal Rejuvenation , Thin Endometrium, Libido Enhancement, Urinary Incontinence and other forms of Gynaecological aesthetic treatments. It appears PRP works very well, in order to help restore healthy vaginal tissue. Reports from many other experts from around the globe indicate enhanced sensitivity of the genital tissues leading to an enhanced enjoyable and emotional sexual experience.
Helping Pregnancies come true…
Haven’t been able to witness the joy of becoming a mother even after all the strenuous attempts? Lost all hopes due to recurrent unsuccessful IVF and Implantation? Or have seen the face of failure or cancellation every time you’re prepared for Transfers due to unready endometrium? Have all these failed attempts made you wondered why? No its nothing close to a curse, just a medical uncertainty that can be looked after.
A healthy endometrium lining has a significant role to offer- it determines IVF success rate as well as implantation success after transfer. Therefore, in order to ensure a health endometrium it is important we take a glance at some of the reasons unsupportive of the same.
Prime Cause for a Thin Endometrium
The reasons behind an improper endometrium lining can be one to many; some of the common ones are as follows::
- Low estrogen levels and poor blood flow to the uterus have been the two sweeping reasons in a lot of cases.
- Conventional therapies resulting in Uterine Infections and inflammations have been a common cause for an unresponsive endometrium as well. Such events are relatively frequent in Women who have previously underwent trauma of the uterus like previous cesarean sections, repetitive curettage or have been affected by Asher man’s syndrome or have suffered from chronic infections, endometritis, pelvic inflammatory disease and malposition of uterus.
- Low estradiol values and excessive use of Clomiphene Citrate are amongst the few other causes.
- Fibrosis of Uterus is yet another condition which occurs during acute or chronic infections. The fibrosis initiates at the base of the tissue and leads into the destruction of the basal layer of the endometrium, further resulting in narrowing of the uterine cavity. The deterioration of basal layer, makes the regeneration of endometrium really hard further accelerating the problem to a great extent.
- Various Uterine Surgeries, such as reiterated curettage, intracavitary myomectomy and polypectomy might result in intrauterine adhesions later damaging the endometrium via the same mechanism as fibrosis.
- Highly intensive cancer treatments, such as radical surgery, chemotherapy and radiotherapy (RT) can permanently alter present or future reproductive ability in both men and women.
Stem Cell Treatment for Thin Endometrium
It is said to believe that an endometrium thickness of less than 7 mm is considered as thin endometrium and such a condition negatively impacts the IVF success rate. Quoting the pregnancy as unlikely in such a case.
However, if there’s an uncertainty that persists, there’s a solution to it too and here, the solution to this problem is Intrauterine Infusion of Platelet Rich Plasma., we’ve seen the bar rise in terms of affectivity of PRP therapy for thin endometrium lining during the studies and treatments conducted so far.
PRP therapy is a powerful weapon that helps in the rejuvenation of the uterine lining thus improving its ability to perform all the roles that it is supposed to facilitate. One of the major roles being improving the Implantation success rate and as per the diagnosis made so far, a good thick endometrium lining and an adequate endometrial receptivity are the two main factors influencing implantation success and leading into a successful pregnancy after the embryo transfer.
We have results that highlight how a thin endometrium lining during IVF affects implantation and is the cause behind the failure of the embryo implantation after transfer. Hence, this is the
reason why transfers are cancelled so frequently if the endometrium lining doesn’t achieve the required thickness.
PRP (Platelet Rich Plasma) contains a group of activating platelets that prompts the action of cytokines and growth factors collectively- ensuing the regulation of cell migration, attachment, proliferation & differentiation, also promoting the accumulation of extracellular matrix within the cell. Thus, as per the theory, local infusion of PRP may enhance endometrial receptivity and implantation through naturally recurring stages.
Benefits of Stem Cell Therapy For Thin Endometrium:
A recently conducted study clearly demonstrates that PRP increases the proliferation not only on cultured fibroblasts, but has a profound effect on mesenchymal cells as well, which happen to be the progenitors of different types of cells, including endometrial cells. Henceforth, this evidence stands positively in our support , embracing the claim that PRP can stimulate some of the cellular processes involved in endometrial regeneration thus making it an effective treatment therapy for the administration of a thin endometrium lining.
Based on the recorded evidences as per the conducted studies five patients who volunteered to undergo the therapy responded with successful thickening of endometrium following intra uterine infusion of PRP and all of them happen to conceive.
Asherman syndrome is a rare, acquired condition of the uterus. It is a uterine disorder in which scar tissue and adhesion form within the uterus due to trauma, surgeries, infections.
In severe cases, the entire front and back walls of the uterus can merge together. In milder cases, the adhesions can formin smaller areas of the uterus. These adhesions can be thick or thin, and may be sparsely located or merged together depending upon the situations.
Intrauterine Adhesion prevent implantation of blastocysts, weaken the blood supply to the early fetus, and thus results in pelvic pain, Recurrent miscarriages and infertility.
Stem Cell Treatment for Asherman syndrome
It has been witnessed that stem cell therapy proved to be the best treatment for ashrman syndrome. Stem cells have been successfully used to treat arious blood born conditions like myeloma, leukemia, lymphoma. A pool of stem cell isolated from potent sources such as bone marrow, adipose tissues.
These stem cell have the ability to regenerate endometrial lining to a very great extent.
It has been evidenced improved menstruation, lower pelvic pain, adequate growth in endometrial lining after taking stem cell therapy.